Western blot: 0.5-1ug/ml
Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
0.5mg/ml if reconstituted with 0.2ml sterile DI water
This CCL4 antibodyis to be used only for research purposes and not for diagnostics..
Rat partial recombinant protein (AA 24-92) was used as the immunogen for this CCL4 antibody.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
The stated application concentrations are suggested starting amounts. Titration of the CCL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
After reconstitution, the CCL4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Chemokine (C-C motif) ligand 4 is a protein which in human is encoded by the CCL4 gene. By analysis of somatic cell hybrids and by in situ hybridization, the gene was assigned to 17q21-q23. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize CCL4. Based on functional analysis, one or more additional beta chemokine receptors that transduce CCL4 signals must also exist. MIP-1alpha and CCL4 have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).