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Anti-CCL4 (Polyclonal), ALEXA Fluor 594

Basic information

Name

Anti-CCL4 (Polyclonal), ALEXA Fluor 594

size

100 microliters

Catalog number

GENTObs-2475R-A594

Price

489 €

Details and extended information

Subcellular locations

N/A

Swiss Prot

N/A

Gene ID

6351

Target Protein/Peptide

CCL4

Modification site

None

Applications

IF(IHC-P)

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated

Alexa conjugate 1

Conjugated with

ALEXA FLUOR® 594

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

Clonality

Polyclonal Antibody

Other name

Anti-CCL4 Polyclonal

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Also known as

CCL4 Polyclonal Antibody

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

Specificity

This antibody reacts specifically with CCL4

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from mouse CCL4

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

Synonyms

MIP-1 Beta; Macrophage Inflammatory Protein 1 beta; MIP-1 Beta; MIP-1β; Secreted protein G 26; ACT 2; ACT2; AT744.1; CC chemokine ligand 4; CCL4; ccl4l 1; CCL4L1; Chemokine CC Mot Ligand 4; G 26 T lymphocyte secreted protein; HC21; LAG 1; LAG1; Lymphocyte activation gene 1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1 beta; MGC104418; MGC126025; MGC126026; MIP1 beta; MIP1B; MIP1B1; PAT 744; Protein H400; SCYA2; SCYA4; Secreted protein G26; SIS gamma; Small inducible cytokine A4; T cell activation protein 2; CCL4_MOUSE;

Background information

MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.