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MIP-1 beta (human) ELISA Kit

Basic information

Name

MIP-1 beta (human) ELISA Kit

size

100 assays

Catalog number

K4785-100

Price

909 €

Details and extended information

Species reactivity

human

Storage condition

-20°C

Shipping condition

gel pack

Maximum time can be stored

12 months

Category

MIP-1 Beta

Kit's other name

Macrophage Inflammatory Protein-1 beta (human) ELISA Kit,

Samples tested

• Serum & plasma • Cell culture supernatants • Urine

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Applications

Quantitative protein detection, establishing normal range, validation of antibody array results

Highlights

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

Contents

• MIP-1 β antibody-coated Microplate (Item A), 96 wells • Wash Buffer (20x) (Item B) • rh MIP-1 β Standards (Item C) • Assay Diluent A (Item D) • Assay Diluent B (5x) (Item E) • MIP-1 β Detection Antibody (Item F) • HRP-Streptavidin (600x) (Item G) • TMB (Item H) • Stop Solution (Item I)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Kit's benefits

• Easy, convenient and time-saving method to assay MIP-1 beta. • The minimum detectable concentration of MIP-1 beta is less than 2.5 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF).

Kit's description

BioVision’s Human MIP-1 beta (MIP-1 β) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 β. This assay employs an antibody specific for human MIP-1 β coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 β present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 β antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 β bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF). The minimum detectable dose of MIP-1 β is typically less than 2.5 pg/ml. Detection Range: 2.5 pg/ml – 1,000 pg/ml. The intra-Assay reproducibility is CV