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anti-MIP-1a Antibody [1.2_3E8-2H6-2B6]

Basic information

Name

anti-MIP-1a Antibody [1.2_3E8-2H6-2B6]

size

0.5 mg

Catalog number

XP-5734-M

Price

180 €

Details and extended information

NCBI GENE nr

6348

GENE symbol

CCL3

Works with

Human

Ab raised in

Mouse

Omim nr

127078

Protein nr

P10147

Ncbi nr

P10147

French translation

anticorps

Type

Monoclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Monoclonal

1.2_3E8-2H6-2B6

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot, NELISAut

Antigene name

chemokine (C-C motif) ligand 3

Purity

This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.

Antigen

The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1a; as the immunizing antigen.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene names

Macrophage inflammatory protein 1 alpha, CCL 3, MIP 1 alpha, MIP1a, SCYA 3, Heparin binding chemotaxis protein, G0S19-1, MIP1A, SCYA3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha,

Keep at

MIP-1a; lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.

Gene info

Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Antibody advice

Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1a; (100 ng/mL), a concentration of 5.0-10.0 ug/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1a; when used with our biotinylated antigen affinity purified anti-human MIP-1a; (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/mL.Western Blot:To detect hMIP-1a; by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a; is 1.0-2.0 ng/lane, under non-reducing conditions.