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anti-MIP-1B Antibody

Basic information

Name

anti-MIP-1B Antibody

size

0.1 mg

Catalog number

XP-5235

Price

180 €

Details and extended information

NCBI GENE nr

6351

GENE symbol

CCL4

Works with

Human

Omim nr

127080

Ab raised in

Rabbit

Protein nr

P13236

Ncbi nr

P13236

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot, NELISAut

Antigene name

chemokine (C-C motif) ligand 4

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-1-beta.

Purity

Anti-hMIP-1-beta specific antibody was purified by affinity chromatography employing immobilized hMIP-1-beta matrix.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Gene names

ACT2, G-26, HC21, LAG1, LAG-1, MIP1B, SCYA2, SCYA4, MIP1B1, AT744.1, MIP-1-beta, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

MIP-1B antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Antibody advice

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/mL), a concentration of 1.75 - 3.0 ug/mL of this antibody is required. ELISA:To detect hMIP-1-beta by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1-beta.Sandwich:To detect hMIP-1β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1β (XP-5235Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1β. Western Blot:To detect hMIP-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.