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anti-MIP-1a Antibody

Basic information

Name

anti-MIP-1a Antibody

size

0.05 mg

Catalog number

XP-5234Bt

Price

180 €

Details and extended information

GENE symbol

Ccl3

Works with

Mouse

NCBI GENE nr

20302

Omim nr

127079

Antibodie's label

Biotin

Ab raised in

Rabbit

Protein nr

P10855

Ncbi nr

P10855

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Mus musculus

Immuno application

ELISA, Western Blot

Antigene name

chemokine (C-C motif) ligand 3

Purity

Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-alpha (murine MIP-1-alpha).

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Gene names

Mip1a, Scya3, G0S19-1, AI323804, MIP1-(a), LD78alpha, MIP-1alpha, MIP1-alpha, Mip1a, C-C motif chemokine 3, Heparin-binding chemotaxis protein, MIP-1-alpha

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

MIP-1a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Gene info

MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Antibody advice

ELISA:To detect mMIP-1-a; by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMIP-1-a;.Sandwich:To detect mMIP-1a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MIP-1a; (XP-5234) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1a;. Western Blot:To detect mMIP-1-a; by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-a; is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.