Contact us on 02033938531 or vasil@gentaur.com.

anti-MIP-1a Antibody

Basic information

Name

anti-MIP-1a Antibody

size

0.05 mg

Catalog number

XP-5233Bt

Price

180 €

Details and extended information

Works with

Rat

GENE symbol

Ccl3

NCBI GENE nr

25542

Antibodie's label

Biotin

Ab raised in

Rabbit

Protein nr

P50229

Ncbi nr

P50229

Omim nr

1709024

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Immuno application

ELISA, Western Blot

Antigene name

chemokine (C-C motif) ligand 3

Source

Rattus norvegicustus norvegicus

Purity

Anti-rat MIP-1-alpha specific antibody was purified by affinity chromatography and then biotinylated.

Gene names

Scya3, MIP-1a, Mip1a, Scya3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat MIP-1-alpha (rat MIP-1-alpha).

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

MIP-1a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Gene info

Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Antibody advice

ELISA:To detect rat MIP-1-a; by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ug/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat MIP-1-a;.Sandwich:To detect Rat MIP-1a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat MIP-1a; (XP-5233) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MIP-1a;. Western Blot:To detect rat MIP-1-a; by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat MIP-1-a; is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.