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anti-MIP-1B Antibody

Basic information

Name

anti-MIP-1B Antibody

size

0.05 mg

Catalog number

38-146

Price

180 €

Details and extended information

GENE symbol

Ccl4

Works with

Mouse

NCBI GENE nr

20303

Ncbi nr

P14097

Ab raised in

Rabbit

Protein nr

P14097

Antibodie's label

Biotin

Omim nr

1346534

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Mus musculus

Immuno application

ELISA, Western Blot

Antigene name

chemokine (C-C motif) ligand 4

Gene names

Act-2, Mip1b, Scya4, MIP-1B, AT744.1, C-C motif chemokine 4, Immune activation protein 2, ACT-2

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β. Murine MIP-1β specific antibody was purified by affinity chromatography and then biotinylated.

Keep at

MIP-1B antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Antibody advice

ELISA:Direct:To detect mMIP-1b by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.SandwichTo detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine MIP-1b as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.