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Rat MIP-1 alpha/CCL3 PicoKine ELISA Kit

Basic information

Name

Rat MIP-1 alpha/CCL3 PicoKine ELISA Kit

size

1x96 well plate

Catalog number

EK1219

Price

557 €

Details and extended information

Minutes of TMB incubation

25-30

Entrez nr.

25542

Gen protein identity

P50229

ELISA's sensitivity

<1pg/ml

Gene tested

rat MIP-1 alpha

Antigen

E.coli, A24-A92

Latin name

Rattus norvegicus

ELISA kit detection limits

7.8pg/ml to 500pg/ml

Uniprot

C-C motif chemokine 3

Anti-coagulation

heparin, EDTA or Citrate

Gene name

chemokine (C-C motif) ligand 3

Target of this ELISA

Natural and recombinant rat MIP-1 alpha

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Description

The MIP-1 alpha/CCL3 PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA's application

For quantitative measurement of rat MIP-1 alpha in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

ELISA standard curve

Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.137 + 0.218 + 0.283 + 0.422 + 0.691 + 1.101 + 1.816 + 2.423

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);53;177;293;65;192;317 ELISA standard deviations 2.76;11.86;13.2;4.1;14.6;18.4 CV(%);5.2;6.7;4.5;6.3;7.6;5.8

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml rat MIP-1α standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each rat MIP-1α standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- rat MIP-1 alpha antibodies;1 ST0000-10;Lyophilized recombinant rat MIP-1 alpha standard;10ng/tube×2 AR1107;Biotinylated anti- rat MIP-1 alpha antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Alternative

C C motif chemokine 3;CCL3;CCL3_HUMAN;Chemokine (C C motif) ligand 3;Chemokine ligand 3;G0/G1 switch regulatory protein 19 1;G0/G1 switch regulatory protein 19-1;G0S19 1;G0S19 1 protein;Heparin binding chemotaxis protein;L2G25B;LD78-alpha(4-69);LD78ALPHA;Macrophage inflammatory protein 1 alpha;Macrophage inflammatory protein 1-alpha;MIP 1 alpha;MIP-1-alpha;MIP-1-alpha(4-69);MIP1A;PAT 464.1;SCYA 3;SCYA3;SIS alpha;SIS beta;SIS-beta;Small inducible cytokine A3;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein

ELISA kit description

GENTAUR's rat MIP-1 alpha ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for MIP-1 alpha was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, A24-A92) and your samples are measured with a biotin conjugated polyclonal from goat specific for MIP-1 alpha will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat MIP-1 alpha in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

More gene info

Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.