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Rat CCL4/MIP-1 beta PicoKine ELISA Kit

Basic information

Name

Rat CCL4/MIP-1 beta PicoKine ELISA Kit

size

1x96 well plate

Catalog number

EK0939

Price

557 €

Details and extended information

Minutes of TMB incubation

15-20

Entrez nr.

116637

Gen protein identity

P50230

Gene tested

Rat CCL4

ELISA's sensitivity

<10pg/ml

Antigen

E.Coli, A24-N92

Latin name

Rattus norvegicus

Uniprot

C-C motif chemokine 4

ELISA kit detection limits

15.6pg/ml to 1000pg/ml

Anti-coagulation

heparin, EDTA or citrate

Gene name

chemokine (C-C motif) ligand 4

Target of this ELISA

Natural and recombinant rat CCL4

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

ELISA's application

For quantitative measurement of rat CCL4 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.005 + 0.112 + 0.234 + 0.450 + 0.835 + 1.179 + 1.667 + 2.174

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);35;112;367;36;116;371 ELISA standard deviations 1.3;6.1;21.3;1.9;7.4;26.7 CV(%);3.7;5.4;5.8;5.3;6.4;7.2

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Alternative

ACT-2;ACT2;AT744.1;C-C motif chemokine 4;CC chemokine ligand 4;CCL4;CCL4_HUMAN;G-26 T-lymphocyte-secreted protein;HC21;LAG-1;LAG1;Lymphocyte activation gene 1 protein;Macrophage inflammatory protein 1-beta;MIP-1-beta;MIP-1-beta(1-69);MIP-1-beta(3-69);MIP1-B;MIP1B;MIP1B1;PAT 744;Protein H400;SCYA2;SCYA4;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2

How the elisa works

Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml rat CCL4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each rat CCL4 standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- rat CCL4 antibodies;1 ST0000-10;Lyophilized recombinant rat CCL4 standard;10ng/tube×2 AR1107;Biotinylated anti- rat CCL4 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

More gene info

Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).

ELISA kit description

GENTAUR's rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibodies from mouse specific for CCL4 was used as capure antibodies on the ELISA test plate. The internal standards (E.Coli, A24-N92) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffers. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffers. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CCL4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.