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Human CCL4/MIP-1 beta PicoKine ELISA Kit

Basic information

Name

Human CCL4/MIP-1 beta PicoKine ELISA Kit

size

1x96 well plate

Catalog number

EK0450

Price

510 €

Details and extended information

Entrez nr.

6351

Minutes of TMB incubation

25-30

Gen protein identity

P13236

ELISA's sensitivity

<1pg/ml

Gene tested

Human CCL4

Antigen

E.coli, A24-N92

ELISA kit detection limits

7.8pg/ml to 500pg/ml

Uniprot

C-C motif chemokine 4

Anti-coagulation

heparin, EDTA or citrate

Gene name

chemokine (C-C motif) ligand 4

Target of this ELISA

Natural and recombinant human CCL4

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

ELISA's application

For quantitative measurement of human CCL4 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

ELISA standard curve

Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.016 + 0.058 + 0.110 + 0.197 + 0.423 + 0.756 + 1.247 + 1.807

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);32;91;324;33;87;319 ELISA standard deviations 1.3;3.8;15.2;2.1;5.8;24.9 CV(%);4.1;4.2;4.7;6.4;6.7;7.8

Alternative

ACT 2;ACT2;AT744.1;C C motif chemokine 4;CCL4;CCL4L1;Chemokine CC Motif Ligand 4;G 26 T lymphocyte-secreted protein;HC21;LAG 1;LAG1;Lymphocyte activation gene 1;Lymphocyte activation gene 1 protein;MGC104418;MGC126025;MGC126026;MIP 1 beta;MIP1B;PAT 744;Protein H400;SCYA2;SCYA4;Secreted protein G 26;SIS gamma;Small inducible cytokine A4;T cell activation protein 2

More gene info

Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human CCL4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each human CCL4 standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- human CCL4 antibodies;1 ST0000-10;Lyophilized recombinant human CCL4 standard;10ng/tubex2 AR1107;Biotinylated anti- human CCL4 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA kit description

GENTAUR's human CCL4 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for CCL4 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, A24-N92) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL4 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human CCL4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.