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Mouse CCL3/MIP1 alpha PicoKine ELISA Kit

Basic information

Name

Mouse CCL3/MIP1 alpha PicoKine ELISA Kit

size

1x96 well plate

Catalog number

EK0449

Price

510 €

Details and extended information

Minutes of TMB incubation

20-25

Entrez nr.

20302

Gen protein identity

P10855

ELISA's sensitivity

<10pg/ml

Gene tested

murine CCL3

Latin name

Mus musculus

Antigen

E.coli, A24-A92

ELISA kit detection limits

7.8pg/ml to 500pg/ml

Uniprot

C-C motif chemokine 3

Anti-coagulation

heparin, EDTA or citrate

Gene name

chemokine (C-C motif) ligand 3

Target of this ELISA

Natural and recombinant mouse CCL3

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Detector and capure antibodies

A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Description

The Mouse CCL3/MIP1 alpha PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

ELISA's application

For quantitative measurement of mouse CCL3 in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 7.8 + 15.6 + 31.3 + 62.5 + 125 + 250 + 500 O.D. + 0.106 + 0.134 + 0.166 + 0.226 + 0.345 + 0.529 + 1.028 + 1.704

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);102;234;421;86;255;437 ELISA standard deviations 3.88;9.6;22.3;4.82;17 31 CV(%);3.8;4.1;5.3;5.6;6.7;7.1

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse CCL3 antibodies;1 ST0000-10;Lyophilized recombinant mouse CCL3 standard;10ng/tubex2 AR1107;Biotinylated anti- mouse CCL3 antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Alternative

C C motif chemokine 3;CCL3;CCL3_HUMAN;Chemokine (C C motif) ligand 3;Chemokine ligand 3;G0/G1 switch regulatory protein 19 1;G0/G1 switch regulatory protein 19-1;G0S19 1;G0S19 1 protein;Heparin binding chemotaxis protein;L2G25B;LD78-alpha(4-69);LD78ALPHA;Macrophage inflammatory protein 1 alpha;Macrophage inflammatory protein 1-alpha;MIP 1 alpha;MIP-1-alpha;MIP-1-alpha(4-69);MIP1A;PAT 464.1;SCYA 3;SCYA3;SIS alpha;SIS beta;SIS-beta;Small inducible cytokine A3;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein

ELISA kit description

GENTAUR's mouse CCL3 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CCL3 was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, A24-A92) and your samples are measured with a biotin conjugated polyclonal from goat specific for CCL3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CCL3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

More gene info

Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.