Contact us on 02033938531 or vasil@gentaur.com.

Human MIP-1 alpha/CCL3 PicoKine ELISA Kit

Basic information

Name

Human MIP-1 alpha/CCL3 PicoKine ELISA Kit

size

1x96 well plate

Catalog number

EK0448

Price

532 €

Details and extended information

Entrez nr.

6348

Minutes of TMB incubation

15-20

Gen protein identity

P10147

ELISA's sensitivity

<10pg/ml

Antigen

E.coli, A27-A92

Gene tested

Human MIP-1 alpha

Uniprot

C-C motif chemokine 3

ELISA kit detection limits

15.6pg/ml to 1000pg/ml

Anti-coagulation

heparin, EDTA or citrate

Gene name

chemokine (C-C motif) ligand 3

Target of this ELISA

Natural and recombinant human MIP-1 alpha

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Description

The MIP-1 alpha/CCL3 PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

ELISA's application

For quantitative measurement of human MIP-1 alpha in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

ELISA standard curve

Concentration(pg/ml) + 0 + 15.6 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 O.D. + 0.136 + 0.218 + 0.306 + 0.472 + 0.734 + 1.090 + 1.758 + 2.233

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);107;450;732;95;413;682 ELISA standard deviations 4.39;13.05;25.62;6.84;22.3;34.78 CV(%);4.1;2.9;3.5;7.2;5.4;5.1

How the elisa works

Sample the amount of 100ul/well of the standard solution at 1000 pg per ml, 500, 25, 125, 62.5, 31.25, 15.6 pg/ml human MIP-1 alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to the ELISA plate. Keep in mind that each human MIP-1 alpha standard solution and each sample be measured in duplicate.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- human MIP-1 alpha antibodies;1 ST0000-10;Lyophilized recombinant human MIP-1 alpha standard;10ng/tubex2 AR1107;Biotinylated anti- human MIP-1 alpha antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

Alternative

C C motif chemokine 3;CCL3;CCL3_HUMAN;Chemokine (C C motif) ligand 3;Chemokine ligand 3;G0/G1 switch regulatory protein 19 1;G0/G1 switch regulatory protein 19-1;G0S19 1;G0S19 1 protein;Heparin binding chemotaxis protein;L2G25B;LD78-alpha(4-69);LD78ALPHA;Macrophage inflammatory protein 1 alpha;Macrophage inflammatory protein 1-alpha;MIP 1 alpha;MIP-1-alpha;MIP-1-alpha(4-69);MIP1A;PAT 464.1;SCYA 3;SCYA3;SIS alpha;SIS beta;SIS-beta;Small inducible cytokine A3;Small-inducible cytokine A3;Tonsillar lymphocyte LD78 alpha protein

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA kit description

GENTAUR's human MIP-1 alpha ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for MIP-1 alpha was used as capure antibodies on the ELISA test plate. The internal standards (E.coli, A27-A92) and your samples are measured with a biotin conjugated polyclonal from goat specific for MIP-1 alpha will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human MIP-1 alpha in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

More gene info

Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5kDa.