MIP-1 alpha Recombinant Protein

Rat

25542

P50229

E. coli

40254794

Lyophilized

NP_037157.2

recombinants

Rattus norvegicus

Recombinants or rec. proteins

chemokine (C-C motif) ligand 3

This product is for research use only.

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The MIP-1 alpha Recombinant Protein is a α- or alpha protein sometimes glycoprotein present in blood.

Scya3, MIP-1a, Mip1a, Scya3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological activity was determined by its ability to chemoattract rat peritoneal macrophages using a concentration of 50.0 - 100.0 ng/mL.

The lyophilized MIP-1 alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted MIP-1 alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing MIP-1 alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells. Recombinant rat MIP-1 alpha is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.