anti-MIP-1a Antibody [1.2_3E8-2H6-2B6]
ELISA, Western Blot, NELISAut
chemokine (C-C motif) ligand 3
This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.
The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1a; as the immunizing antigen.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Macrophage inflammatory protein 1 alpha, CCL 3, MIP 1 alpha, MIP1a, SCYA 3, Heparin binding chemotaxis protein, G0S19-1, MIP1A, SCYA3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha,
MIP-1a; lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 grades C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4 grades C when reconstituted.
Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1a; (100 ng/mL), a concentration of 5.0-10.0 ug/mL of this antibody is required.ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 ug/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1a; when used with our biotinylated antigen affinity purified anti-human MIP-1a; (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/mL.Western Blot:To detect hMIP-1a; by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1a; is 1.0-2.0 ng/lane, under non-reducing conditions.