ELISA, Western Blot, NELISAut
chemokine (C-C motif) ligand 23
Anti-hMIP-3 specific antibody was purified by affinity chromatography employing immobilized hMIP-3 matrix.
CKb8, MIP3, Ckb-8, MIP-3, MPIF-1, SCYA23, Ckb-8-1, hmrp-2a, CK-BETA-8, MPIF1, C-C motif chemokine 23, CK-beta-8, CKB-8
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3 (human Macrophage Inflammatory Protein-3).
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
MIP-3 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3 (100.0 ng/mL), a concentration of 2.0 - 4.0 ug/mL of this antibody is required. ELISA:To detect hMIP-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3.Sandwich:To detect hMIP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-3 (XP-5238Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3. Western Blot:To detect hMIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.