Human MIP-1β (Macrophage Inflammatory Protein 1 Beta) ELISA Kit

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Human

MIP-1β

Sandwich

Ice packs

4.5 hours

18.75 pg/mL

Colorimetric

31.25-2000 pg/mL

6-11 business days

Serum, Plasma, Biological Fluids

Short term: 4°C; Long term: see manual.

For research use only. Not for diagnostic procedures.

The kit is manufactured at ISO 9001 certified facilities.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

No significant cross-reactivity or interference was observed.

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Use Human MIP-1β (Macrophage Inflammatory Protein 1 Beta) ELISA Kit before 6 months

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-1β . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIP-1β. The concentration of Human MIP-1β in samples can be calculated by comparing the OD of the samples to the standard curve.