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anti-MIP-1a Antibody

Basic information

Name

anti-MIP-1a Antibody

size

0.1 mg

Catalog number

XP-5232

Price

180 €

Details and extended information

Ab raised in

Goat

NCBI GENE nr

6348

GENE symbol

CCL3

Works with

Human

Omim nr

127078

Protein nr

P10147

Ncbi nr

P10147

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot, NELISAut

Antigene name

chemokine (C-C motif) ligand 3

Antigen

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-1 alpha.

Gene names

MIP1A, SCYA3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha

Purity

Anti-hMIP-1 alpha specific antibody was purified by affinity chromatography employing immobilized hMIP-1 alpha matrix.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

MIP-1a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Gene info

MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Antibody advice

Neutralization: Neutralization data available. ELISA:To detect hMIP-1 alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-1 alpha. Sandwich:To detect hMIP-1a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-1a; (XP-5232Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1a;.Western Blot:To detect hMIP-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.