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Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit

Basic information

Name

Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit

size

96Test

Catalog number

E-CL-R0431-96T

Price

541 €

Details and extended information

UNIProt ID

NA

Target Species

Rat

Target Name

MIP-1α

Type

Sandwich

Sensitivity

4.69pg/mL

Detection Type

Colormetric

Detection Range

7.81~500pg/mL

Latin name

Rattus norvegicus

Tested Sample Types

Serum, Plasma, Cell supernatant

Target Synonym

CCL3, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, G0S19-1

Product Name

Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit

Description

The MIP-1α (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MIP-1α . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MIP-1α , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MIP-1α . You can calculate the concentration of Rat MIP-1α in the samples by comparing the RLU value of the samples to the standard curve.